Research Article

Developed and validated HPLC method for simultaneous analysis of key flavonoids and phenolcarboxylic acids in the hawthorn-based cardiotonic Forticor®

Authors: Vanja Tadic (Institute for Medicinal Plants Research “Dr. Josif Pančić”, Belgrade, Serbia) , Dragica Bojovic (University of Donja Gorica, Podgorica, Faculty for Food Technology, Food Safety and Ecology, Montenegro) , Goran Marković (Zvezdara Clinical Hospital Center, Belgrade, Serbia) , Ivana Nešić (University of Nis, Faculty of Medicine, Niš, Serbia) , Ana Žugić (Institute for Medicinal Plants Research “Dr. Josif Pančić”, Belgrade, Serbia)

  • Developed and validated HPLC method for simultaneous analysis of key flavonoids and phenolcarboxylic acids in the hawthorn-based cardiotonic Forticor®

    Research Article

    Developed and validated HPLC method for simultaneous analysis of key flavonoids and phenolcarboxylic acids in the hawthorn-based cardiotonic Forticor®

    Authors: , , , ,

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Abstract

Relatively simple, but very reliable, one-step qualitative/quantitative HPLC analysis of four key flavonoids, and a phenolcarboxylic acid, has been introduced. For substrate/sample preparations, either triple percolations, using 70% ethanol-water (V/V), or/and ultrasonic bath methanol extractions, were carried out to obtain the desired isolates yielding quercetin, isoquercitrin, hyperoside, vitexin and chlorogenic acid. The method is linear, over the studied range of 1.05 – 210.00, 6.25 – 250.00, 25.00 – 250.00, 7.50 – 150.00 and 1.04 – 10.40 μg/mL for chlorogenic acid, vitexin, hyperoside, isoquercitrin and quercetin, respectively. The correlation coefficient for each of the analytes was greater than 0.999. The intra-day and inter-day precision of the analysis was below 2.00 and 3.00 %, respectively. The accuracy of the analysis is verified by the standard addition method, using three different concentrations of each component in the tested materials, with recovery values obtained in the range of 98.04 – 102.47% (RSD ≤ 1.85%). The detection limits were 0.6, 0.5, 0.5, 0.8 and 0.3 μg/mL chlorogenic acid, vitexin, hyperoside, isoquercitrin and quercetine, respectively. The developed method is convenient in the routine control of hawthorn raw materials and pharmaceutical dosage forms made from hawthorn berries or flower-bearing branches. The new HPLC method may be used for the quality control of the commercially used cardiotonic Forticor® intended for increasing/strengthening cardiac muscles, in order to lower the risks of atherosclerosis, hypertension and congestive heart failure.

Keywords: Hawthorn, extraction, HPLC method, validation, forticor®

How to Cite:

Tadic, V., Bojovic, D., Marković, G., Nešić, I. & Žugić, A., (2023) “Developed and validated HPLC method for simultaneous analysis of key flavonoids and phenolcarboxylic acids in the hawthorn-based cardiotonic Forticor®”, Lekovite Sirovine 43(1), 1-6. doi: https://doi.org/10.61652/leksir2343e171t

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Published on
2023-12-25

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