Research Article

Inhibition of quorum sensing virulente factors of Pseudomonas aeruginosa PAO1 by Ferulago macedonica and Echinophora sibthorpiana extracts and essential oils

Authors: Ksenija Mileski (University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade, Serbia) , Ana Ćirić (University of Belgrade, Institute for Biological Research “Siniša Stankovic”-National Institute of Republic of Serbia, Department of Plant Physiology, Belgrade, Serbia) , Vlado Matevski (University “S. Kiril and Metodij”, Faculty of Natural Sciences and Mathematics, Institute of Biology, Skopje, North Macedonia) , Petar Marin (University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade, Serbia) , Marina Soković (University of Belgrade, Institute for Biological Research “Siniša Stankovic”-National Institute of Republic of Serbia, Department of Plant Physiology, Belgrade, Serbia) , Ana Džamić (University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade, Serbia)

  • Inhibition of quorum sensing virulente factors of Pseudomonas aeruginosa PAO1 by Ferulago macedonica and Echinophora sibthorpiana extracts and essential oils

    Research Article

    Inhibition of quorum sensing virulente factors of Pseudomonas aeruginosa PAO1 by Ferulago macedonica and Echinophora sibthorpiana extracts and essential oils

    Authors: , , , , ,

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Abstract

This study was conducted to analyse extracts and essential oils of Ferulago macedonica and Echinophora sibthorpiana (Apiaceae) for anti–quorum sensing potential using Pseudomonas aerugonisa PAO1 as biomonitoring system. Anti–quorum sensing screening was carried out by tree different tests which showed that examined samples possessed significant effects in suppressing of some virulent determinants of PAO1. The essential oils of F . macedonica and E . sibthorpiana aerial parts reduced the growth and modified the appearance of P . aeruginosa colonies and flagella to a large extent in twitching and motility assay. In the presence of essential oil of E . sibthorpiana , diameter of the colony was 6.67 mm, the flagella were absent and the color of the colony was changed from green to white. In anti–biofilm assay, the inhibitory activity of E . sibthorpiana was more notable than of F . macedonica . Applied colorimetric test revealed that examined samples strongly reduced the production of pigment pyocyanin where the highest inhibition of its synthesis showed F . macedonica oil (5.22%), followed by E . sibthorpiana oil (10.69%), which was singnificantly bigger potential in comparison to used antibiotics (141.55%).

Keywords: Apiaceae, Ferulago macedonica, Echinophora sibthorpiana, anti–quorum sensing, Pseudomonas aeruginosa PAO1

How to Cite:

Mileski, K., Ćirić, A., Matevski, V., Marin, P., Soković, M. & Džamić, A., (2017) “Inhibition of quorum sensing virulente factors of Pseudomonas aeruginosa PAO1 by Ferulago macedonica and Echinophora sibthorpiana extracts and essential oils”, Lekovite Sirovine 37(1), 33-40. doi: https://doi.org/10.5937/leksir1737033m

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Published on
2017-12-25

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