Abstract
In this study, water and various concentrations of ethanol in water (30 %, 50 %, 70 %, and 90 %) were used as a solvent in the extraction of two different samples of hemp ( Cannabis sativaL.). The extraction yield, total phenols content, total flavonoids content, antioxidant activity, and reductive capacity were determined in the obtained extracts. The extraction yield was from 8.16 to 19.56 %, the content of total phenols was in the range from 5.85 to 17.05 mg GAE/g dw, and content of total flavonoids was in the range from 5.85 to 9.25 mg CE/g dw. Antioxidant activity was tested by DPPH assay and EC50 values were from 0.1331 to 0.7563 mg/mL, while EC50 values obtained by reducing power test ranged from 0.4450 to 1.1980 mg/mL. Ethanol/water mixture (50 %) was determined to be the best solvent for the extraction of phenolic compounds from both hemp samples. Total phenols content in 50 % ethanolic extracts were 17.05 mg/g dw and 9.25 mg/g dw for young and mature hemp, respectively.
Keywords: cannabis, phenols, Antioxidant activity, flavonoids
How to Cite:
Drinić, Z., Vidović, S., Vladić, J., Koren, A., Kiprovski, B. & Sikora, V., (2018) “Effect of extraction solvent on total polyphenols content and antioxidant activity of Cannabis sativa L.”, Lekovite Sirovine 38(1), 17-21. doi: https://doi.org/10.5937/leksir1838017d
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