Research Article
Authors: Katarina Šavikin (Institute for Medicinal Plants Research „Dr. Josif Pančić“, Belgrade, Serbia) , Ana Alimpić (University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Belgrade, Serbia) , Gordana Zdunić (Institute for Medicinal Plants Research „Dr. Josif Pančić“, Belgrade, Serbia) , Jelena Živković (Institute for Medicinal Plants Research „Dr. Josif Pančić“, Belgrade, Serbia) , Teodora Janković (Institute for Medicinal Plants Research „Dr. Josif Pančić“, Belgrade, Serbia) , Nebojša Menković (Institute for Medicinal Plant Research “Dr. Josif Pančić”, Belgrade, Serbia) , Sonja Duletić-Laušević (University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”Belgrade, , Serbia)
Different biological activities of Hypericum perforatum L. dry extract was tested in this study. Antioxidant activity was evaluated by four different assays. Antineurodegenerative activity was tested using acetylcholinesterase (AChE) and tyrosinase inhibitory activity assays. In all four antioxidant assays, concentration dependent manner was noticed in the activity of tested extract. The most active was the highest applied concentration (500 µg/ml). In DPPH test, extract applied in concentration of 200 µg/ml showed similar or higher radical scavenging activity compared to the concentration of 100 µg/ml applied for standard substances BHA and BHT. The lowest antioxidant activity for the extract was detected in ABTS test. All applied concentrations of dry extract were significantly more potent than vitamin C in β-carotene test. Moreover, the extract reached 40.31 and 45.56% of the inhibition in AChE and TYR assays, respectively. In both cases, the most active concentration of the extract was 200 µg/ml.
Keywords: Hypericum perforatum, dry extract, biological activity
How to Cite: Šavikin, K. , Alimpić, A. , Zdunić, G. , Živković, J. , Janković, T. , Menković, N. & Duletić-Laušević, S. (2017) “Antioxidant and antineurodegenerative properties of St. John’s-wort dry extract”, Lekovite Sirovine. 37(1). doi: https://doi.org/10.5937/leksir1737005s