Research Article
Authors: Zorica Drinić (Institute for Medicinal Plants Research “Dr. Josif Pančić”, Belgrade, Serbia) , Senka Vidović (University of Novi Sad, Faculty of Tehnology, Novi Sad, Serbia) , Jelena Vladić (University of Novi Sad, Faculty of Tehnology, Novi Sad, Serbia) , Anamarija Koren (Institute of Field and Vegetable Crops, Novi Sad, Serbia) , Biljana Kiprovski (Institute of Field and Vegetable Crops, Belgrade, Serbia) , Vladmir Sikora (Institute of Field and Vegetable Crops, Novi Sad, Serbia)
In this study, water and various concentrations of ethanol in water (30 %, 50 %, 70 %, and 90 %) were used as a solvent in the extraction of two different samples of hemp ( Cannabis sativaL.). The extraction yield, total phenols content, total flavonoids content, antioxidant activity, and reductive capacity were determined in the obtained extracts. The extraction yield was from 8.16 to 19.56 %, the content of total phenols was in the range from 5.85 to 17.05 mg GAE/g dw, and content of total flavonoids was in the range from 5.85 to 9.25 mg CE/g dw. Antioxidant activity was tested by DPPH assay and EC50 values were from 0.1331 to 0.7563 mg/mL, while EC50 values obtained by reducing power test ranged from 0.4450 to 1.1980 mg/mL. Ethanol/water mixture (50 %) was determined to be the best solvent for the extraction of phenolic compounds from both hemp samples. Total phenols content in 50 % ethanolic extracts were 17.05 mg/g dw and 9.25 mg/g dw for young and mature hemp, respectively.
Keywords: cannabis, phenols, Antioxidant activity, flavonoids
How to Cite: Drinić, Z. , Vidović, S. , Vladić, J. , Koren, A. , Kiprovski, B. & Sikora, V. (2018) “Effect of extraction solvent on total polyphenols content and antioxidant activity of Cannabis sativa L.”, Lekovite Sirovine. 38(1). doi: https://doi.org/10.5937/leksir1838017d