Assessment of antioxidant and hepatoprotective potential of Satureja montana extracts against CCl4 induced liver damage

Tatjana Ćebović, Jelena Vladić, Aleksandra Gavarić, Zoran Zeković, Senka Vidović



The influence of Satureja montana extracts on several liver biochemical parameters in mice administered with carbon tetrachloride was estimated. In vivo investigation of antioxidant properties of S. montana extracts encompassed monitoring of biochemical parameters (derived from liver homogenate and blood hydrolyzate) and examination of potential hepatoprotective effect after intoxication with carbon tetrachloride. Biochemical tests included determination of activity of several antioxidant enzymes: xanthine oxidase, catalase, peroxidase, glutathione peroxidase, amount of reduced glutathione and intensity of lipid peroxidation. In order to obtain data about the potent hepatoprotective effect of examined extracts, the following parameters were determined: aspartate aminotransferase, alanine aminotransferase, bilirubin, hepatic DNA and hydroxyprolin. Results of this study demonstrate that S. montana extracts inhibited the hepatotoxicity produced by carbon tetrachloride administration most probably through activation of physiological common defense mechanisms.


Satureja montana; hepatoprotective; antioxidant

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Aeschbach, R., Löliger, J., Scott, B. C., Murcia, A., Butler, J., Halliwell, B., Aruoma O. I. (1994). Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Food Chem. Toxicol. 32(1), 31-36.

Aristatile B., Al-Numair K.S., AlAssaf A., Veeramani C., Pugalendi K.V. (2015). Protective effect of carvacrol on oxidative stress and cellular DNA damage induced by UVB irradiation in human peripheral lymphocytes. J. Biochem Mol Toxic. 29, 497-507.

Aristatile, B., Al‐Numair, K. S., Veeramani, C., Pugalendi, K. V. (2009). Effect of carvacrol on hepatic marker enzymes and antioxidant status in d‐galactosamine‐induced hepatotoxicity in rats. Fundam. Clin. Pharmacol. 23(6), 757-765.

Assaei, R., Zal, F., Mostafavi-Pour, Z., Dabbaghmanesh, M. H., Geramizadeh, B., Omrani, G. H. R., Pajouhi, N. (2014). Hepatoprotective effect of Satureja khuzestanica essential oil and vitamin E in experimental hyperthyroid rats: evidence for role of antioxidant effect. I.J.M.S. 39(5), 459.

Beers, R. F., Sizer, I. W. (1952). A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem. 195(1), 133-140.

Bergmayer, U. H. (1970). Methods of enzymatic analysis. Weinheim: Verlag Chemie; 483-484.

Beuthler, E., Duron, O., Kelly, B. (1983). Improved methods for the determination of blood glutathione. J. Lab. Clin. Med. 61, 882-889.

Beutler, E. (1984). Red cell metabolism: a manual of biochemical methods. Grune & Stratton, 3rd edn. University Press: London.

Blokhina, O., Virolainen, E., Fagerstedt, K. V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 91(2), 179-194.

Buege, J. A., Aust, S. D. (1978). Microsomal lipid peroxidation. Methods in Enzymology; 52, 302-310.

Četojević-Simin, D.D., Čanadanvić-Brunet, J.M., Bogdanović, G.N., Ćetković, G.S., Tumbas, V.T., Djilas, S.M. (2004). Antioxidative and antiproliferative effects of Satureja montana L. extracts. J. BUON. 9, 443-449.

Ćavar, S., Maksimović, M., Šolić, M.E., Jerković-Mujkić, A., Bešta, R. (2008). Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils. Food Chem. 111, 648-653.

Ćavar, S., Šolić, M.E., Maksimović, M. (2013). Chemical composition and antioxidant activity of two Satureja species from Mt. Biokovo. Botanica Serbica. 37, 159-165.

Ćebović, T., Maksimović, Z. (2012). Hepatoprotective effect of Filipendula hexapetala Gilib. (Rosaceae) in carbon tetrachloride‐induced hepatotoxicity in rats. Phytother. Res. 26(7), 1088-1091.

El Tawab, A. M. A., Shahin, N. N., Abdel Mohsen, M. M. (2014). Protective effect of Satureja montana extract on cyclophosphamide-induced testicular injury in rats. Chem. Biol. Interac. 224, 196-205.

Espín, J. C., Soler-Rivas, C., Wichers, H. J. (2000). Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2, 2-diphenyl-1-picrylhydrazyl radical. J. Agric. Food Chem. 48(3), 648-656.

Goldberg, D. M., Spooner, R. J., 1983. Glutathione reductase. In: Methods of enzymatic analysis, Bergmayer HU., Eds, Weinheim: Basel; PP 258-265.

Grosso, C., Oliveira, A.C., Mainar, A.M., Urieta, J.S., Barroso, J.G., Palavra, A.M.F. (2009). Antioxidant activities of the supercritical and conventional Satureja montana extracts. Journal Food Sci. 74, C713-C717.

Guimarães A.G., Oliveira G.F., Melo M.S., Cavalcanti S.C., Antoniolli A.R., Bonjardim L.R., Silva F.A., Santos J.P., Rocha R.F., Moreira J.C., Araújo A.A., Gelain D.P., Quintans-Júnior L.J. (2010). Bioassay guided evaluation of antioxidant and antinociceptive activities of carvacrol. Basic & Clinic. Pharmacol. Toxicol., 107, 949-957.

Gutierrez, R. M. P., Navarro, Y. T. G. (2010). Antioxidant and hepatoprotective effects of the methanol extract of the leaves of Satureja macrostema. Phcog. Mag. 6(22), 125.

Manna, P., Sinha, M., Sil, P. C. (2006). Aqueous extract of Terminalia arjuna prevents carbon tetrachloride induced hepatic and renal disorders. BMC Complement. Altern. Med. 6(1), 33.

Merlin, N. J., Parthasarathy, V. (2011). Antioxidant and hepatoprotective activity of chloroform and ethanol extracts of Gmelina asiatica aerial parts. J. Med. Plants Res. 5(4), 533-538.

Prieto, J.M., Iacopini, P., Cioni, P., Chericoni, S. (2007). In vitro activity of the essential oils of Origanum vulgare, Satureja montana and their main constituents in peroxynitrite-induced oxidative processes. Food Chem. 104, 889-895.

Schulte-Hermann, R., Ochs, H., Bursch, W., Parzefall, W. (1988). Quantitative structure-activity studies on effects of sixteen different steroids on growth and monooxygenases of rat liver. Cancer Res. 48(9), 2462-2468.

Serrano, C., Matos, O., Teixeira, B., Ramos, C., Neng, N., Nogueira, J., Nunes, M.L., Marques, A. (2011). Antioxidant and antimicrobial activity of Satureja montana L. extracts. J. Sci. Food Agr. 91, 1554-1560.

Silva, F. V., Martins, A., Salta, J., Neng, N. R., Nogueira, J. M., Mira, D., Gaspar, N., Justino, J., Grosso, C., Urieta, J., Palavra, A. M. Rauter, A. (2009). Phytochemical profile and anticholinesterase and antimicrobial activities of supercritical versus conventional extracts of Satureja montana. J. Agric. Food Chem. 57(24), 11557-11563.

Simon, L. M., Fatrai, Z., Jonas, D. E., Matkovics, B. (1974). Study of peroxide metabolism enzymes during the development of Phaseolus vulgaris. Biochem. Physiol. Pfl. 166(5), 387-392.

Skočibušić, M., Bezić, N. (2004). Phytochemical analysis and in vitro antimicrobial activity of two Satureja species essential oils. Phytother. Res. 18, 967-970.

Slamenova D., Horvathova E., Chalupa I., Wsolova L., Navarova J. (2010). Ex vivo assessment of protective effects of carvacrol against DNA lesions induced in primary rat cells by visible light excited methylene blue (VL+ MB). Neoplasma, 58, 14-19.

Sreelatha, S., Padma, P. R., Umadevi, M. (2009). Protective effects of Coriandrum sativum extracts on carbon tetrachloride-induced hepatotoxicity in rats. Food Chem. Toxicol. 47(4), 702-708.

Stanić, G., Samaržija, I. (1993). Diuretic Activity of Satureja montana subsp. montana extracts and oil in rats. Phytother. Res. 7, 363-366.

Suntres, Z. E., Coccimiglio, J., Alipour, M. (2015). The bioactivity and toxicological actions of carvacrol. Crit. Rev. Food Sci. Nutr. 55(3), 304-318.

Thabrew, M. I., Joice, P. D. T. M., Rajatissa, W. (1987). A comparative study of the efficacy of Pavetta indica and Osbeckia octandra in the treatment of liver dysfunction. Planta Med. 53(03), 239-241.

Ultee, A., Bennik, M. H. J., Moezelaar, R. (2002). The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus. Appl. Environ. Microbiol. 68(4), 1561-1568.

Vidović, S., Vladić, J., Vaštag, Z., Zeković, Z., Popović, L. (2014). Maltodextrin as a carrier of health benefit compounds in Satureja montana dry powder extract obtained by spray drying technique. Powder Technol. 258, 209-215.

Vladić, J., Canli, O., Pavlić, B., Zeković, Z., Vidović, S., Kaplan, M. (2017a). Optimization of Satureja montana subcritical water extraction process and chemical characterization of volatile fraction of extracts. J. Supercrit. Fluids. 120 (2017) 86-94.

Vladić, J., Vidović, S., Aćimović, M., Gavarić, A., Jokić, S. (2017b). Satureja montana: Cultivation, Production and Uses. In Medicinal Plants: Production, Cultivation and Uses. Matthias A, Laisné N. (ed). New York, USA: Nova Science Publishers, 27-57.

Vladić, J., Zeković, Z., Jokić, S., Svilović, S., Kovačević, S., Vidović, S. (2016). Winter savory: Supercritical carbon dioxide extraction and mathematical modeling of extraction process. J. Supercrit. Fluids. 117, 89-97.

Woessner, J. F. (1961). The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch. Biochem. Biophys. 93(2), 440-447.

Yamasaki, K., Nakano, M., Kawahata, T., Mori, H., Otake, T., Ueba, N., Oishi, I., Inami, R., Yamane, M., Nakamura, M., Murata, H., Nakanishi, T. (1998). Anti-HIV-1 activity of herbs in Labiatae. Biol. Pharm. Bull. 21, 829-833.

Yang, J., Li, Y., Wang, F., Wu, C. (2010). Hepatoprotective effects of apple polyphenols on CCl4-induced acute liver damage in mice. J. Agric. Food Chem. 58(10), 6525-6531.


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