IN VITRO ANTITUMORAL ACTIVITY OF THE EXTRACT OF SPONGE ACANTHELLA ACUTA

Tatjana Stanojković, Sanja Milović, Ivana Matić, Nađa Grozdanić, Zoran Kljajić

DOI: http://dx.doi.org/10.5937/leksir1535089S

Abstract


Seaweeds are an excellent source of compounds with biological activity.Particularly interesting are the sponges, which suited in medicine reaches into the distant past. The biological effects of extracts and new compounds from sponges have been reported in numbers of scientific papers. Sponges produce a plethora of chemical compounds with widely varying carbon skeletons, which have been found to interfere with pathogenesis at many different points. Due to this, sponges have the potential to provide future drugs against important diseases, such as cancer. All of this makes them particularly interesting to examine the antitumor activity. In this paper for the first time presented a data of investigations of antitumor activity of extract of sponge Acanthella acuta, in vitro. Crude samples of Acanthella acuta (phylum Porifera) were collected from the natural locality in Adriatic Sea, the Bay of Kotor, and subjected to extraction. After that, we examined the cytotoxicity and cell cycle distribution of dichloromethane/methanol (1:1) extract of Acanthella acuta, on two human malignant cell lines, human cervix carcinoma (HeLa)and human colon carcinoma (LS174): and also a normal fetal lung fibroblast cell line (MRC5). The IC50 values in the MTT assay in LS174 and HeLa cells were ranged from 9.92±0.54 to 29.51±μg/ml. Moreover, cytotoxic activity of Acanthella acuta extract on normal MRC5 cells was not observed. Cell cycle distribution was quantified by flow cytometry. In vitro antitumor activites was accompained by an important subG1 accumulation of HeLa cells after treatment of tested cell lines with extract.

Keywords


HeLa cells, Acanthella acuta, cytotoxic activity, MTT assay, cell cycle

Full Text:

PDF

References


Abraham, I., el Sayed, K., Chen, Z., Guo, H. (2012) Current Status on Marine Products with Reversal Effect on Cancer Multidrug Resistance. Marine Drugs, 10(12): 2312-2321

Angerhofer, C.K., Pezzuto, J.M., König, G.M., Wright, A.D., Sticher, O. (1992) Antimalarial activity of sesquiterpenes from the marine sponge Acanthella klethra. Journal of natural products, 55(12): 1787-9

Aoki, S., Chen, Z., Higasiyama, K., Setiawan, I., Akiyama, S., Kobayashi, M. (2001) Reversing Effect of Agosterol A, a Spongean Sterol Acetate, on Multidrug Resistance in Human Carcinoma Cells. Japanese Journal of Cancer Research, 92(8): 886-895

Aoki, S., Cao, L., Matsui, K., Rachmat, R., Akiyama, S., Kobayashi, M. (2004) Kendarimide A, a novel peptide reversing P-glycoprotein-mediated multidrug resistance in tumor cells, from a marine sponge of Haliclona sp. Tetrahedron, 60(33): 7053-7059

Ashour, M.A., Elkhayat, E.S., Rainer, E., Edrada, R.A. (2007) Indole alkaloid from the Red Sea sponge Hyrtios erectus. Arkivoc, 2007(15): 225

Beedessee, G., Ramanjooloo, A., Tiscornia, I., Cresteil, T., Raghothama, S., Arya, D., Rao, S., Gowd, K.H., Bollati-Fogolin, M., Marie, D.E. (2014) Evaluation of hexane and ethyl acetate extracts of the sponge J aspis diastra collected from Mauritius Waters on HeLa cells. Journal of Pharmacy and Pharmacology, 66(9): 1317-1327

Bergquist, R.P. (1978) Sponges. Berkeley: Universiti of Califronia Press

Blunt, J.W., Copp, B.R., Keyzers, R.A., Munro, M.H.G., Prinsep, M.R. (2013) Marine natural products. Natural product reports, 30(2): 237-323

Blunt, J.W., Copp, B.R., Keyzers, R.A., Munro, M.H.G., Prinsep, M.R. (2014) Marine natural products. Natural product reports, 31(2): 160-258

Brown, J.W., Cappell, S., Perez-Stable, C., Fishman, L.M. (2004) Extracts from two marine sponges lower cyclin B1 levels, cause a G2/M cell cycle block and trigger apoptosis in SW-13 human adrenal carcinoma cells. Toxicon, 43(7): 841-846

Chairman, K., Singh, A.R., Alagumuthu, G. (2012) Cytotoxic and antioxidant activity of selected marine sponges. Asian Pacific Journal of Tropical Disease, 2(3): 234-238

Chakraborty, C., Hsu, C.H., Wen, Z.H., Lin, C.S. (2019) Anticancer drugs discovery and development from marine organism. Curr Top Med Chem., 9(16); 1536-45

Chen, Z., Aoki, S., Komatsu, M., Ueda, K., Sumizawa, T., Furukawa, T., Okumura, H., Ren, X., Belinsky, M.G., Lee, K., Kruh, G.D., Kobayashi, M., Akiyama, S. (2001) Reversal of drug resistance mediated by multidrug resistance protein (MRP) 1 by dual effects of agosterol a on MRP1 function. International Journal of Cancer, 93(1): 107-113

Clothier, R.H. (1995) The FRAME cytotoxicity test. Methods Mol Biol, 43: 109-18

Ferreira, E.G., Wilke, D.V., Jimenez, P.C., i dr. (2007) Cytotoxic activity of hydroethanolic extracts of sponges (Porifera) collected at Pedra da Risca do Meio Marine State Park. u: Ceará State, Porifera Research: Biodiversity, Innovation and Sustainability, Brazil, 313-318

Gottesman, M.M., Fojo, T., Bates, S.E. (2002) Multidrug resistance in cancer: Role of ATP-dependent transporters. Nature reviews. Cancer, 2(1): 48-58

Laport, M., Santos, O., Muricy, G. (2009) Marine Sponges: Potential Sources of New Antimicrobial Drugs. Current Pharmaceutical Biotechnology, 10(1): 86-105

Lopez, D., Martinez-Luis, S. (2014) Marine Natural Products with P-Glycoprotein Inhibitor Properties. Marine Drugs, 12(1): 525-546

Márquez, F.M.D., Acosta, L.M.E., Márquez, F.M.E., Martínez, M.A., Márquez, F.E.J., Camargo, G.M. (2012) Effect of extracts from the calcareous sponge Leucetta aff. floridana on the cell cycle of leukemoid cell lines. Rev Cubana Farm., 46(4); 436-445

Mayer, A.M.S., Glaser, K.B. (2013) Marine Pharmacology and the Marine Pharmaceuticals Pipeline. FASEB Journal, 27: 7; 1167

Miyaoka, H., Nishijima, S., Mitome, H., Yamada, Y. (2000) Three New Scalarane Sesterterpenoids from the Okinawan Sponge Hyrtios erectus. Journal of Natural Products, 63(10): 1369-1372

Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2): 55-63

Narasimharaju, A., Thameemulansari, L.H., Maheswara, U.C. (2012) Cytotoxic activity of methanol and dichloromethane extracts from marine sponges. International Journal of Pharmacy and Pharmaceutical Sciences, 4(3): 277-279

Ohno, M., Abe, T. (1991) Rapid colorimetric assay for the quantification of leukemia inhibitory factor (LIF) and interleukin-6 (IL-6). Journal of immunological methods, 145(1-2): 199-203

Quesada, A.R., Grávalos, M.G., Puentes, J.F. (1996) Polyaromatic alkaloids from marine invertebrates as cytotoxic compounds and inhibitors of multidrug resistance caused by P-glycoprotein. British Journal of Cancer, 74(5): 677-682

Ramachandran, M., Titus, I., Backyavathy, P.M., Nambikkairaj, B. (2013) In vitro determination of marine sponge Hyrtios erectus secondary metabolite effect against human breast and larynx cancer cell lines. International Journal of Current Research, 5(2); 124-128

Shi, Z., Jain, S., Kim, I., Peng, X., Abraham, I., Youssef, D.T.A., Fu, L., El, S.K., Ambudkar, S.V., Chen, Z. (2007) Sipholenol A, a marine-derived sipholane triterpene, potently reverses P-glycoprotein (ABCB1)-mediated multidrug resistance in cancer cells. Cancer science, 98(9): 1373-80


Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Lekovite Sirovine



ISSN 0455-6224 (Print)
ISSN 2560-3965 (Online)

Creative Commons License Except where otherwise noted, the content on this site is licensed under a Creative Commons Attribution 4.0 International License.