Tatjana Stanojković, Sanja Milović, Ivana Matić, Nađa Grozdanić, Zoran Kljajić



Seaweeds are an excellent source of compounds with biological activity.Particularly interesting are the sponges, which suited in medicine reaches into the distant past. The biological effects of extracts and new compounds from sponges have been reported in numbers of scientific papers. Sponges produce a plethora of chemical compounds with widely varying carbon skeletons, which have been found to interfere with pathogenesis at many different points. Due to this, sponges have the potential to provide future drugs against important diseases, such as cancer. All of this makes them particularly interesting to examine the antitumor activity. In this paper for the first time presented a data of investigations of antitumor activity of extract of sponge Acanthella acuta, in vitro. Crude samples of Acanthella acuta (phylum Porifera) were collected from the natural locality in Adriatic Sea, the Bay of Kotor, and subjected to extraction. After that, we examined the cytotoxicity and cell cycle distribution of dichloromethane/methanol (1:1) extract of Acanthella acuta, on two human malignant cell lines, human cervix carcinoma (HeLa)and human colon carcinoma (LS174): and also a normal fetal lung fibroblast cell line (MRC5). The IC50 values in the MTT assay in LS174 and HeLa cells were ranged from 9.92±0.54 to 29.51±μg/ml. Moreover, cytotoxic activity of Acanthella acuta extract on normal MRC5 cells was not observed. Cell cycle distribution was quantified by flow cytometry. In vitro antitumor activites was accompained by an important subG1 accumulation of HeLa cells after treatment of tested cell lines with extract.


HeLa cells, Acanthella acuta, cytotoxic activity, MTT assay, cell cycle

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