Dragana Četojević-Simin, Ivana Beara, Nataša Simin



The goal of this work was to introduce the parameter for the precise evaluation of in vitroanti-tumor activity of Plantago altissima, P. lanceolata, P. reniformis, Allium flavum, A. cepa, Tuber aestivum i T. magnatumextracts.For the evaluation of the cell growth human tumor cell lines HeLa, MCF7 and HT-29 were used, as well as cell line derived from the healthy tissue (MRC-5). The cell growth was evaluated using colorimetric sulforhodamine B (SRB) assay.NT/T IC50 parameter gives precise information on the in vitro anti-tumor efficiency of evaluated extracts. The highest efficiency was achieved using water extracts of black and white truffle towards breast adenocarcinoma , reaching extremely high NT/T values (NT/T=200-500). The efficiency of several methanolic extracts of truffles was high (NT/T=21,33-70): while the efficiency of all Plantago sp.extracts was moderate (NT/T=3,24-4,27) towards breast adenocarcinoda and cervix carcinoma. The least efficient were extracts of aerial parts of A. flavum that have been more cytotoxic towards healthy cells than to colon adenocarcinoma (NT/T=0,23) and cervix carcinoma (NT/T=0,75).Extremely high NT/T valus that were obtained using water extracts of black and white truffle recommend their use in the anti-tumor therapy and suggest their use in health prevention and in the production of nutraceuticals and food supplements.


Anti-tumor acitivity; in vitro; natural products; Plantago sp.; Allium sp.; Tuber sp.

Full Text:



Anackov, G. (2009) Taxonomy and chorology of genus Allium L. 1754 (Amaryllidales, Alliaceae). University of Novi Sad, in Serbia

Burja, A.M., Banaigs, B., Abou-Mansour, E., Burgess, J. G., Wright, P.C. (2001) Marine cyanobacteria—a prolific source of natural products. Tetrahedron, 57(46): 9347-9377

Ceruti, A., Fontana, A., Nosenzo, C. (2003) Le Specie Europee del genere Tuber - Una Revisione Storica. Torino: Museo Regionale de Scienze Naturali

Cragg, G.M., Newman, D.J., Weiss, R.B. (1997) Coral reefs, forests, and thermal vents: the worldwide exploration of nature for novel antitumor agents. Seminars in oncology, 24(2): 156-63

Četojević-Simin, D.D., Armaković, S.J., Šojić, D.V., Abramović, B.F. (2013) Toxicity assessment of metoprolol and its photodegradation mixtures obtained by using different type of TiO2 catalysts in the mammalian cell lines. Science of The Total Environment, 463-464: 968-974

Četojević-Simin, D.D., Velićanski, A.S., Cvetković, D.D., Markov, S.L., Ćetković, G.S., Tumbas-Šaponjac, V.T., Vulić, J.J., Čanadanović-Brunet, J.M., Djilas, S.M. (2015) Bioactivity of Meeker and Willamette raspberry (Rubus idaeus L.) pomace extracts. Food Chemistry, 166: 407-413

Grlić, L. (1986) Enciklopedija samoniklog jestivog bilja. Zagreb: August Cesarec

Hall, I.R., Brown, G.T., Zambonelli, A. (2007) Taming the truffle: The history, lore, and science of the ultimate mushroom. Portland: Timber Press

Harvey, A. (2000) Strategies for discovering drugs from previously unexplored natural products. Drug Discovery Today, 5(7): 294-300

Marjanović, Ž., Grebenc, T., Marković, M., Glišić, A., Milenković, M. (2010) Ecological specificities and molecular diversity of truffles (genus Tuber) originating from mid-west of the Balkan Peninsula. Sydowia, 62: 67-87

Montecchi, A., Sarasini, M. (2000) Funghi ipogei d'Europa. Trento-Vicenza: Associaazione Micologi ca Bresadola - Fondacione centro Studi Micologici

Redzic, S.J. (2006) Wild Edible Plants and Their Traditional Use in the Human Nutrition in Bosnia‐Herzegovina. Ecology of Food and Nutrition, 45(3): 189-232

Ronsted, N., Franzyk, H., Molgaard, P., Jaroszewski, J. W., Jensen, S. R. (2003) Chemotaxonomy and evolution of Plantago L. Plant Systematics and Evolution, 242(1-4): 63-82

Simin, N., Orcic, D., Četojević-Simin, D.D., Mimica-Dukic, N., Anackov, G., Beara, I., Mitic-Culafic, D., Bozin, B. (2013) Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae). LWT - Food Science and Technology, 54(1): 139-146

Skehan, P., Storeng, R., Scudiero, D., Monks, A., McMahon, J., Vistica, D., Warren, J. T., Bokesch, H., Kenney, S., Boyd, M. R. (1990) New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening. Journal of the National Cancer Institute / JNCI, 82(13): 1107-1112

Strohl, W.R. (2000) The role of natural products in a modern drug discovery program. Drug Discovery Today, 5(2): 39-41


  • There are currently no refbacks.

Copyright (c) 2017 Lekovite Sirovine

ISSN 0455-6224 (Print)
ISSN 2560-3965 (Online)

Creative Commons License Except where otherwise noted, the content on this site is licensed under a Creative Commons Attribution 4.0 International License.