Miloš Nikolić, Jasmina Glamočlija, Ana Ćirić, Tatjana Marković, Dejan Marković, Tamara Perić, Marina Soković


The aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils of peppermint Mentha piperita L. GC / MS techniques confirmed the presence of the 48 components of which menthol (35.57 %) and menthone (22.50 %) were the most abundant. Antimicrobial activity, ie the minimum inhibitory and minimum bactericidal / fungicidal (MIC, MBC/MFC) concentrations were obtained using the mikordilution method against pathogenic microorganism isolated from the oral cavity (8 bacteria and 58 Candida sp.) and referent strains. The essential oil of peppermint showed good antimicrobial activity with MIC from 0.63 to 5 mg / ml and 0.13 to 0.5 for bacteria and fungi and MBC 1.25 to 10 mg / ml , ie. MFC 0.5-1 mg / ml. Compared to HexoralÒ, peppermint essential oil has a very strong antimicrobial potential. The results of these tests give a new value, which will enrich the knowledge of peppermint oil od presented chemical composition, opens the door for further research towards its use in dentistry and as well in prevention and treatment of various diseases caused by tested pathogens.


essential oil; peppermint; Mentha piperita L.,; antimicrobial activity.

Full Text:



Hornok, L. (1992). “Cultivation and Processing of Medicinal Plants”, L. Hornok, Ed., John Wiley & Sons, 187–196.

İşcan G., N. Kirimer, M. Kurkcuoglu, K.H.C. Baser, F. Demirci (2002). Antimicrobial screening of Mentha piperita essential oils. Journal of Agricultural Food Chemistry, 50(14): 3943–3946.

Hammer, K.A., Carson, C.F., Riley, T.V. (1999). „Antimicrobial activity of essential oils and other plant extracts“, Journal of Applied Microbiology, 86(6): 985– 990.

Kizil S., Haşimi N., Tolan V., Kilinç E., Yüksel U. (2010). “Mineral content, essential oil components and biological activity of two mentha species (M. piperita L., M. spicata L.)”, Turkish Journal of Field Crops, 15(2): 148–153.

Agarwal V., Lal P., Pruthi V. (2008). “Prevention of Candida albicans biofilm by plant oils”, Mycopathologia, 165(1): 13–19.

Adams, R. P. (2009). (IV ed.) Allured Publishing Corporation, Carol Stream, IL, USA.

Cecchini, C., Silvi, S., Cresci, A., Piciotti, A., Caprioli, G., Papa, F., Sagratini, G., Vittori, S., Maggi, F. (2012). “Antimicrobial efficacy of Achillea ligustica All. (Asteraceae) essential oils against reference and isolated oral microorganisms”, Chemistry and Biodiversity, 9(1): 12–24.

Nikolić, M., Glamočlija, J., Ćirić, A., Perić, T., Marković, D., Stević, T., Soković, M. (2012). “Antimicrobial activity of ozone gas and colloidal silver against oral microorganisms”, Digest Journal of Nanomaterials and Biostructures, 7(4): 1693–1699.

Douk, K.D., Dagher, M.S., Sattout, J.E. (1995). “Antifungal activity of the essential oil of Origanum syriacum L.”, J. Food.Prot. 58: 1147-1149.

European Committee on Antibiotic Susceptibility. (2002). European Society of Clinical Microbiology and Infectious Diseases, Taufkirchen, Germany.

Saharkhiz M. J., Motamedi M., Zomorodian K., Pakshir K., Miri R., Hemyari K. (2012). “Chemical composition, antifungal and antibiofilm activities of the essential oil of Mentha piperita L.“, ISRN Pharmaceutics, pp. 1-6.

Lambert, P. A. (2002). „Mechanisms of antibiotic resistance in Pseudomonas aeruginosa“, Journal of the Royal Society of Medicine, 95(41): 22-26.

Vaughn, S. F., Spencer, G. F. (1994). “Antifungal activity of natural compounds against thiabendazole-resistant Fusarium sambucinum strains”, Journal of Agriculture and Food Chemistry, 42: 200−203.

Xianfei X., Xiaoqiang C., Shunying Z., Guolin Z. (2007). “Chemical composition and antimicrobial activity of essential oils of Chaenomeles speciosa from China”, Food Chemistry, 100(4):1312–1315.


  • There are currently no refbacks.

Copyright (c) 2017 Miloš Nikolić, Jasmina Glamočlija, Ana Ćirić, Tatjana Marković, Dejan Marković, Tamara Perić, Marina Soković

ISSN 0455-6224 (Print)
ISSN 2560-3965 (Online)

Creative Commons License Except where otherwise noted, the content on this site is licensed under a Creative Commons Attribution 4.0 International License.