Polyphenols Extraction From Plant Sources

Aleksandra A. Jovanović

DOI: http://dx.doi.org/10.5937/leksir1737046J


Various conventional (maceration and heat-assisted extraction) and new alternative techniques (ultrasound-assisted extraction and microwave-assisted extraction) have been developed for the extraction of polyphenols compounds from different plant sources. Novel procedures have established with the aim to reduce the extraction time and solvent consumption, as well as to increase polyphenols yield and to improve extract quality. A critical review was conducted to introduce and compare traditional and modern procedures applied for extraction of bioactive polyphenols compounds. This review focuses on the different techniques of polyphenols extraction, discussing their operating conditions, mechanism, choice of particle size and solvent, solid-to-solvent ratio, extraction time, advantages/disadvantages and effectiveness. Finally, potential application of those extraction procedures in polyphenols isolation is reviewed.


extraction; polyphenols; particle size; solvents; solid-to-solvent ratio; time

Full Text:



Alessandro, L.G., Kriaa, K., Nikov, I. and Dimitrov, K. (2012). Ultrasound assisted extraction of polyphenols from black chokeberry. Separation and Purification Technology 93, 42-47.

Ali, I.B.E.H., Bahri, R., Chaouachi, M., Boussaïd, M. and Harzallah-Skhiri, F. (2014). Phenolic content, antioxidant and allelopathic activities of various extracts of Thymus numidicus Poir. organs. Industrial Crops and Products 62, 188-195.

Asofiei, I., Calinescu, I., Trifan, A., David, J.G. and Gavrila, A.I. (2016). Microwave-Assisted Batch Extraction of Polyphenols from Sea Buckthorn Leaves. Chemical Engineering Communications 203, 1547-1553.

Aziz, S. and Rehman, H. (2008). Studies on the Chemical Constituents of Thymus serpyllum. Turkish Journal of Chemistry 32, 605-614.

Bhatt, V., Sharma, S., Kumar, N., Sharma, U. and Singh, B. (2017). Simultaneous quantification and identification of flavonoids, lignans, coumarin and amides in leaves of Zanthoxylum armatum using UPLC-DAD-ESI-QTOF-MS/MS. Journal of Pharmaceutical and Biomedical Analysis 132, 46-55.

Both, S., Chemat, F. and Strube, J. (2014). Extraction of polyphenols from black tea – Conventional and ultrasound assisted extraction. Ultrasonic Sonochemistry 21, 1030-1034.

Bouras, M., Chadni, M., Barba, F., Grimi, N., Bals, O. and Vorobiev, E. (2015). Optimization of microwave-assisted extraction of polyphenols from Quercus bark. Industrial Crops and Products 77, 590-601.

Bucić-Kojić, A., Planinić, M., Tomas, S., Mujić, I., Bilić, M. and Velić, D. (2007). Study of solid-liquid extraction kinetics of total polyphenols from grape seeds. Journal of Food Engineering 81, 236-242.

Bucić-Kojić, A., Planinić, M., Tomas, S., Mujić, I., Bilić, M. and Velić, D. (2011). Effect of Extraction Conditions on the Extractability of Phenolic Compounds from Lyophilised Fig Fruits (Ficus Carica L.). Polish Journal of Food and Nutrition Sciences 61, 195-199.

Çam, M. and Hişil, Y. (2010). Pressurised water extraction of polyphenols from pomegranate peels. Food Chemistry 123, 878-885.

Chizzola, R., Michitsch, H. and Franz, C. (2008). Antioxidative Properties of Thymus vulgaris Leaves: Comparison of Different Extracts and Essential Oil Chemotypes. Journal of Agricultural and Food Chemistry 56, 6897-6904.

Chupin, L., Maunu, S.L., Reynaud, S., Pizzi, A., Charrier, B. and Charrier-EL Bouhtoury, F. (2015). Microwave assisted extraction of maritime pine (Pinus pinaster) bark: Impact of particle size and characterization. Industrial Crops and Products 65, 142-149.

Costa, P., Gonçalves, S., Valentão, P., Andrade, P., Coelho, N. and Romano, A. (2012). Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity. Food Chemistry 135, 1253-1260.

Ćujić, N., Šavikin, K., Janković, T., Pljevljakušić, D., Zdunić, G. and Ibrić, S. (2016). Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chemistry 194, 135-142.

Čadanović-Brunet, J., Đilas, S., Ćetković, G., Tumbas, V., Mandić, A. and Čadanović, V. (2006). Antioxidant activities of different Teucrium montanum L. extracts. International Journal of Food Science and Technology 41, 667-673.

Dahmoune, F., Nayak, B., Moussi, K., Remini, H. and Madani, K. (2015). Optimization of microwave-assisted extraction of polyphenols from Myrtus communis L. leaves. Food Chemistry 166, 585-595.

Dandena, A., Zuka, L. and Kostova, M. (2014). Natural antioxidants in black chokeberry marc extracts depending on the extraction method. Foodbalt 2014, 324-326.

Deng, Y., Zhao, Y., Padilla-Zakour, O. and Yang, G. (2015). Polyphenols, antioxidant and antimicrobial activities of leaf and bark extracts of Solidago canadensis L. Industrial Crops and Products 74, 803-809.

Dent, M., Dragović-Uzelac, V., Penić, M., Brnčić, M., Bosiljkov, T. and Levaj, B. (2013). The Effect of Extraction Solvents, Temperature and Time on the Composition and Mass Fraction of Polyphenols in Dalmatian Wild Sage (Salvia officinalis L.) Extracts. Food Technology and Biotechnology 51, 84-91.

Fecka, I. and Turek, S. (2008). Determination of polyphenolic compounds in commercial herbal drugs and spices from Lamiaceae: thyme, wild thyme and sweet marjoram by chromatographic techniques. Food Chemistry 108, 1039-1053.

Horžić, D., Režek Jambrak, A., Belščak-Cvitanović, A., Komes, D. and Lelas, V. (2012). Comparison of Conventional and Ultrasound Assisted Extraction Techniques of Yellow Tea and Bioactive Composition of Obtained Extracts. Food and Bioprocess Technology 5, 2858-2870.

Hossain, M.B., Barry-Ryan, C., Martin-Diana, A.B. and Brunton, N.P. (2011). Optimisation of accelerated solvent extraction of antioxidant compounds from rosemary (Rosmarinus officinalis L.), marjoram (Origanum majorana L.) and oregano (Origanum vulgare L.) using response surface methodology. Food Chemistry 126, 339-346.

Jovanović, A., Đorđević, V., Zdunić, G., Pljevljakušić, D., Šavikin, K., Gođevac, D. and Bugarski, B. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted extraction. Separation and Purification Technology 179, 369-380.

Khan, M.K., Abert-Vian, M., Fabiano-Tixier, A.S., Dangles, O. and Chemat, F. (2010). Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chemistry 119, 851-858.

Lan-Sook, L., Namhyouck, L., Young Ho, K., Chang-Ho, L., Sang, P.H., Yeo-Won, J. and Young-Eon, K. (2013). Optimization of Ultrasonic Extraction of Phenolic Antioxidants from Green Tea Using Response Surface Methodology. Molecules 18, 13530-13545.

Milošević, S., Lepojević, Ž., Zeković, Z. and Vidović, S. (2011). Determination of extraction conditions of Ginkgo biloba L. leaves by supercritical CO2 using response surface methodology. Hemijska industrija 65, 147-157.

Milutinović, M., Radovanović, N., Ćorović, M., Šiler-Marinković, S., Rajilić-Stojanović, M. and Dimitrijević-Branković, S. (2015). Optimisation of microwave-assisted extraction parameters for antioxidants from waste Achillea millefolium dust. Industrial Crops and Products 77, 333-341.

Miron, T.L., Plaza, M., Bahrim, G., Ibáñez, E. and Herrero, M. (2011). Chemical composition of bioactive pressurized extracts of Romanian aromatic plants. Journal of Chromatography A 1218, 4918-4927.

Munin, A. and Edwards-Lévy, F. (2011). Encapsulation of Natural Polyphenolic Compounds; a Review. Pharmaceutics 3, 793-829.

Mustafa, A. and Turner, C. (2011). Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review. Analytica Chimica Acta 703, 8-18.

Nayak, B., Dahmoune, F., Moussi, K., Remini, H., Dairi, S., Aoun, O. and Khodir, M. (2015). Comparison of microwave, ultrasound and accelerated-assisted solvent extraction for recovery of polyphenols from Citrus sinensis peels. Food Chemistry 187, 507-516.

Oniszczuk, A. and Podgórski, R. (2015). Influence of different extraction methods on the quantification of selected flavonoids and phenolic acids from Tilia cordata inflorescence. Industrial Crops and Products 76, 509-514.

Paz, J.W., Márquez, D.M., Ávila, G.M., Cerda, R.B. and Aguilar, C. (2015). Ultrasound-assisted extraction of polyphenols from native plants in the Mexican desert. Ultrasonics Sonochemistry 22, 474-481.

Pinelo, M., Fabbro, P., Manzocco, L., Nuñez, M.J. and Nicoli, M.C. (2005). Optimization of continuous phenol extraction from Vitis vinifera byproducts. Food Chemistry 92, 109-117.

Pompeu, D.R., Silva, E.M. and Rogez, H. (2009). Optimization of the solvent extraction of phenolic antioxidants from fruits of Euterpe oleracea using Response Surface Methodology. Bioresource Technology 100, 6076-6082.

Sen, S. and Chakraborty, R. (2011). The Role of Antioxidants in Human Health, in: Andreescu, S. and Hepel, M., Oxidative Stress: Diagnostics, Prevention, and Therapy, American Chemical Society, Washington, USA, pp. 1-37.

Simić, V., Rajković, K., Stojičević, S., Veličković, D., Nikolić, N., Lazić, M. and Karabegović, I. (2016). Optimization of microwave-assisted extraction of total polyphenolic compounds from chokeberries by response surface methodology and artificial neural network. Separation and Purification Technology 160, 89-97.

Uma, D.B., Ho, C.W. and Wan Aida, W.M. (2010). Optimization of Extraction Parameters of Total Phenolic Compounds from Henna (Lawsonia inermis) Leaves. Sains Malaysiana 39, 119-128.

Vajić, U.-J., Grujić-Milanović, J., Živković, J., Šavikin, K., Gođevac, D., Miloradović, Z., Bugarski, B. and Mihailović-Stanojević, N. (2015). Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology. Industrial Crops and Products 74, 912-917.

Vergara-Salinas, J., Pérez-Jiménez, J., Torres, J.L., Agosin, E. and Pérez-Correa, J. (2012). Effects of Temperature and Time on Polyphenolic Content and Antioxidant Activity in the Pressurized Hot Water Extraction of Deodorized Thyme (Thymus vulgaris). Journal of Agricultural and Food Chemistry 60, 10920-10929.

Vuleta, G., Milić, J. and Savić, S. (2012). Farmaceutska tehnologija, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia (in Serbian).

Wang, L. and Weller, C. (2006). Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology 17, 300-312.

Yang, Y. and Zhang, F. (2008). Ultrasound-assisted extraction of rutin and quercetin from Euonymus alatus (Thunb.) Sieb. Ultrasonics Sonochemistry 15, 308-313.


  • There are currently no refbacks.

Copyright (c) 2017 Aleksandra A. Jovanović

ISSN 0455-6224 (Print)
ISSN 2560-3965 (Online)

Creative Commons License Except where otherwise noted, the content on this site is licensed under a Creative Commons Attribution 4.0 International License.