Inhibition of microbial biofilm formation by Cydonia oblonga Mill. fruit peel and leaf ethanolic extracts

Dejan Stojković, Kristina Jevremović, Marija Smiljković, Jelena Živković, Marina Soković



This study aimed to investigate the activity of C. oblonga peel and leaf ethanolic extracts on biofilm formation of different microbial strains, including pathogenic bacteria and food poisoning strains. It was shown that both of the investigated extracts inhibited biofilm formation in a dose dependent manner with sub-minimal inhibitory concentrations. The percentage of biofilm formation inhibition varied for each bacterial strain and was in the range of 10-100%, for both of the tested extracts. The ability of extracts to inhibit already formed biofilms presented as minimal concentration necessary to disrupt biofilms and concentrations was in the range of 0.010-0.100 mg/mL for the leaf extract and 0.005-0.075 mg/mL for the peel extract. The investigated extracts showed a promising antimicrobial effect comparable to, or even higher than the used reference compounds which make these plant parts attractive for pharmaceutical and food industry.


Cydonia oblonga; biofilm; antibacterial; peel.

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Ashraf, M.U., Muhammad, G., Hussain, M.A. and Bukhari, S.N.A. (2016). Cydonia oblonga M., A medicinal plant rich in phytonutrients for pharmaceuticals. Front Pharmacol. 7, article 163.

Baroni, M.V., Gastaminza, J., Podio N.S., Lingua, M.S., Wunderlin, D.A., Rovasio, J.L., Dotti, R., Rosso, J.C., Ghione, S. and Ribotta, P.D. (2018). Changes in the antioxidant properties of quince fruit (Cydonia oblonga Miller) during jam production at industial scale. J Food Qual. article ID 1460758.

Cerempei, A., Muresan, E.I., Cimpoesu, N., Carp-Carare, C. and Rimbu, C. (2016). Dyeing and antibacterial properties of aqueous extracts from quince (Cydonia oblonga) leaves. Ind Crop Prod. 94, 216-225.

Fattouch, S., Caboni, P., Coroneo, V., Tuberoso, C.I.G., Angioni, A., Dessi, S., Marzouki, N. and Cabras, P. (2007). Antimicrobial activity of tunisian quince (Cydonia oblonga Miller) pulp and peel polyphenols extracts. J Agric Food Chem. 55 (3), 963-969.

Grinan, I., Galindo, A., Rodriguez, P., Morales, D., Corell, M., Centeno, A., Collado-Gonzalez, J., Torrecillas, A., Carbonell-Barrachina, A.A. and Fernandez, F. (2019). Volatile composition and sensory and quality attributes of quince (Cydonia oblonga Mill.) fruits as affected by water stress. Sci Hortic. 244, 68-74.

Karar, M.A.E., Pletzer, D., Jaiswal, R., Weingart, H. and Kuhnert, N. (2014). Identification, characterization, isolation and activity against Escherichia coli of quince (Cydonia oblonga) fruit polyphenols. Food Res Int. 65, 121-129.

Pierce, C.G., Uppuluri, P., Tristan, A.R., Wormley, Jr F.L., Mowat, E., Ramage, G. and Jose, L.L.R. (2008). Nat Protoc. 3, 1494-1450.

Rozalski, M., Micota, B., Sadowska, B., Stochmal, A., Jedrejek, D., Wieckowska-Szakiel, M. and Rozalska, B. (2013). Antiadherent and antibiofilm activity of Humulus lupulus L. derived products: new pharmacological properties. Biomed Res Int, 2013, 101089.

Tolker-Nielsen, T. (2014). Pseudomonas aeruginosa biofilm infections: From molecular biofilm biology to new treatment possibilities. APMIS Suppl. 122 (s138), 1-51.

Williams, P. and Cámara, M. (2009). Quorum sensing and environmental adaptation in Pseudomonas aeruginosa: a tale of regulatory networks and multifunctional signal molecules. Curr. Opin. Microbiol. 12, 182–191.

Zijnge, V., Van Leeuwen, M.B.M., Degener, J.E., Abbas, F., Thurnheer, T., Gmür, R. and Harmsen, H.J.M. (2010). Oral biofilm architecture on natural teeth. PLoS ONE, 5 (2), article number e9321.


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Copyright (c) 2018 Dejan Stojkovic, Kristina Jevremović, Marija Smiljković, Jelena Zivkovic, Marina Sokovic

ISSN 0455-6224 (Print)
ISSN 2560-3965 (Online)

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