The influence of the extraction method on the content of silymarin in Silybi mariani fructus extract

Sofija Đorđević, Teodora Janković, Milica Mihailović

DOI: http://dx.doi.org/10.5937/leksir1838005D

Abstract


This study was aimed to compare the efficiency of silymarin extraction from Silybi mariani fructus using different extraction methods. Maceration, percolation, extraction in a water bath, and ultrasonic-assisted extraction have been performed with 60% ethanol. Extracts were analyzed using UV/Vis and HPLC techniques. Our results showed that the highest concentration of silymarin was detected in samples extracted in a water bath with boiling solvent during 30 and 60 minutes (2.33 and 1.95 %, respectively). Since extraction in a water bath is efficient and reduces a lot of time and solvent consumption, this method could present potential replacement for Pharmacopoeia-recommended Soxhlet extraction procedure.



Keywords


Silybum marianum; silymarin; extraction method; UV spectrophotometry; HPLC.

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Copyright (c) 2018 Sofija Đorđević, Teodora Janković, Milica Mihailović



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