Abstract

The objective of this study was to investigate the influence of enriching the common mushroom species Lentinus edodes with inorganic selenium (Se) at different harvest stages, while comparing two extraction methods and evaluating their in vivo effects on HepG2 liver cells. Selenoprotein (SELENOP) quantification and glutathione peroxidase (GPx) activity were used as key outcome measures. Results indicated that the first flush was more effective, increasing SELENOP levels to 72.4 µg L⁻¹. Enzymatic treatment demonstrated higher efficiency, yielding GPx activities of 62.0 mU mg⁻¹ protein. When comparing treatments, GPx activity was highest during the second flush. Overall, the findings reveal subtle differences related to harvest timing, extraction method, and their impact on both toxicity and anabolic potential in promoting hepatic selenoprotein P biosynthesis. These results suggested that selenium-enriched mushrooms may represent a promising option for dietary supplementation.

Keywords: Selenium-enriched Lentinus edodes, HepG2 cells, Selenoprotein P, glutathione peroxidase

How to Cite:

Milovanovic, I., (2025) “Effects of selenium enriched Lentinus edodes on SELENOP and GPx-1 activity”, Lekovite Sirovine 45(1), 1-4. doi: https://doi.org/10.61652/leksir2545338M